Abstract
The present study aimed to elucidate the underlying mechanism of neuroepithelial cell transforming 1 (NET-1), a member of the Ras homolog gene family, in hepatocellular carcinoma (HCC). To determine the association between the expression of NET-1 and the proliferation and migration of MHCC97-H cells, the cells were transfected with NET-1 small interfering (si)RNA and si negative control. Following transfection with NET-1 siRNA, the proliferation rate of MHCC97-H cells decreased significantly and the percentage of apoptotic cells increased. The HCC cell line MHCC97-H was used in the present study as it exhibited an increased expression level of NET-1 compared with the MHCC97-L cell line. Expression levels of apoptosis-associated proteins including apoptosis regulator Bax (Bax), cyclinD1, apoptosis regulator Bcl-2 (Bcl-2) and caspase-3 were determined. Expression levels of phosphoinositide 3-kinase (PI3K) and protein kinase B (AKT) and their phosphorylated forms were also measured by western blotting. Following NET-1 knockdown, the expression of Bax and cyclinD1 decreased, the expression of Bcl-2 and caspase-3 increased, and the PI3K/AKT signaling pathway was inhibited. The results of the present study suggest that inhibition of NET-1 can suppress the progression of HCC by targeting the PI3K/AKT signaling pathway. NET-1 expression level in HCC cells increased compared with normal liver cells.
Highlights
Hepatocellular carcinoma (HCC) is one of the common malignant tumors worldwide [1]
The results of the reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) assay indicated that the expression levels of neuroepithelial cell transforming 1 (NET‐1) were significantly elevated in MHCC97‐L and MHCC97‐H cells compared with the L‐02 cell line
The MHCC97‐H cell line exhibited the highest expression of NET‐1 among these cell lines (Fig. 1A)
Summary
Hepatocellular carcinoma (HCC) is one of the common malignant tumors worldwide [1]. Globally, there are more than 500,000 new cases each year and about 1 million HCC‐associated casesKey words: neuroepithelial cell transforming 1, hepatocellular carcinoma, proliferation, phosphoinositide 3‐kinase, protein kinase B of mortality [2‐6]. Research on the mechanisms of invasion and metastasis of HCC is important to increase the clinical curative effects and improve the survival rate of patients
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