INHIBITION OF MOBILITY OF NEUTROPHILS OF SURGICAL PATIENTS WITH PURULENT WOUNDS AND WOUND INFECTION AS AN INDICATOR OF INTOXICATION OF THE ORGANISM

Abstract

The method for automatic recording and analysis of motor activity of neutrophils based upon the Magiscan 2A image analysis system allows conducting express diagnostics of motility of neutrophils of patients. It was shown that cells with various speed of movement on glass are contained in the population of neutrophils. The average speed of movement of neutrophil population is a stable indicator of movement activity of the population and can serve as an objective parameter of the functional state of neutrophils with various diseases. Patients with purulent surgical infections manifest correlation between inhibition of neutrophil motility and deteriorating of clinical status of patients (speed in μm / min, healthy donors – 8.8; patients of average severity – 6.5; serious patients – 3.3). Blood serum of serious patients had an inhibitory effect on the migration of neutrophils from healthy donors. Increasing of the average speed of movement of neutrophils from 3.4 to 7.1 μm / min in 1 to 2 days after a radical surgery (amputation) was demonstrated among 11 patients with the diabetic gangrene of the foot. Previously, we have shown the existence of reciprocal relationships between motility of neutrophils and their activation. Inhibition of motility of neutrophils with surgical diseases can be explained by their priming or activation.