Abstract
BackgroundMagnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.ResultsOur results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC50 =11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC50 = 13.6%; v/v) and decreased the amount of melanin (IC50 = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS+ free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.ConclusionsOur results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.
Highlights
Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication
Effect of M. grandiflora L. flower extract on B16F10 cell viability The MTT assay was used to assess the effect of M. grandiflora L. flower extract on B16F10 melanoma cells viability
Effect of M. grandiflora L. flower extract on mushroom tyrosinase activity, B16F10 melanin content and intracellular tyrosinase activity In order to assay the inhibitory effect of the flower extract on mushroom tyrosinase activity, tyrosinase enzyme inhibition experiments were carried out in triplicate
Summary
Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. The inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. The aqueous extracts of flowers and leaves have been reported to exhibit cardiovascular effects [11] and was used as an anticonvulsant in rat [12]. Chemical studies on M. grandiflora L. and other plants of the same genus, such as M. officinalis, have reported the presence of magnolol and honokiol [13,14], which exhibit muscle relaxant activity [15], inhibitory effects on skin tumour promotion [16] and antimicrobial properties [17]. Scientific studies of the antimelanogenic and antioxidant properties of this medicinal plant are lacking
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
