Abstract
Mercuric chloride (HgCl)2 is a toxic metal that causes oxidative damage in several tissues. N-acetylcysteine (NAC) is a sulfhydryl compound with antioxidant activity. In the present study, we investigated the in vitro effects of the association between HgCl2 and NAC in tissues of mice. For this purpose, we evaluated the in vitro effect of HgCl2+NAC association on δ-aminolevulinate dehydratase (δ-ALA-D) activity and on thiobarbituric acid reactive substances (TBARS) levels in liver and kidney of mice. The results demonstrate that HgCl2 inhibited δ-ALA-D activity in both tissues. Hepatic δ-ALA-D activity inhibited by HgCl2 was potentiated by the highest concentration of NAC. The inhibition of hepatic δ-ALA-D activity seems to be related to sulfhydryl groups oxidation of the enzyme. We observed also that HgCl2 increased TBARS levels in kidney and liver. Hepatic TBARS levels were reduced by NAC, at higher concentration. In contrast, NAC, at higher concentration, increased renal TBARS levels. In conclusion, the inhibition of hepatic δ-aminolevulinate dehydratase activity induced by HgCl2 is potentiated by NAC in vitro, and this effect is not related to hepatic lipid peroxidation.
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