Abstract
Epidermal aqueous extracts, previously assayed by their inhibition of epidermal mitosis, were assayed by their inhibition of 3H-thymidine incorporation into epidermal, dermal, and total skin DNA. Several experimental sources for epidermal extract, newborn mouse and cow-snout epidermis, were prepared and assayed with different epidermal and dermal tissues: hairless mouse (hr/hr) and chick embryo shank skin. Cow-snout aqueous epidermal extract inhibits thymidine incorporation into 15- and 19-day-old chick embryo shank skin and mouse skin DNA. The inhibition found in mouse skin DNA was greatest in the epidermis but also present in the dermis. Epinephrine does not influence the inhibition in 15-day-old chick embryo skin. Mouse epidermal extracts inhibit both mouse (hr/hr) epidermal and dermal DNA synthesis. Boiled mouse and cow-snout extracts still inhibit thymidine incorporation into epidermal and dermal DNA. Although tissue specificity of extracts that inhibit epidermal mitosis has previously been demonstrated, our preliminary results with epidermal extracts do not indicate complete tissue specificity when thymidine incorporation into epidermal and dermal DNA is measured. Mouse and cow-snout epidermal extracts inhibit thymidine incorporation into epidermal DNA more than dermal DNA.
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