Inhibition of AMV DNA polymerase by polyriboadenylic acid containing ε-adenosine residues

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Summary Polyriboadenylic acid was treated with chloroacetaldehyde under conditions known to introduce e-adenosine groups. The degree of modification was monitored by increase in fluorescence intensity. Modified e-poly rA was found to be inhibitory when unprimed 70S AMV RNA was used as a substrate, suggesting direct competition with the poly rA tract of the RNA. Since e-poly rA cannot effectively base pair with nucleic acids normally involved in cellular processes, it has the potential of being useful as an inhibitor of oncogenic viral polymerases.