Inhibition effects of commonly used some antibacterial and antiviral drugs on purified human serum paraoxonase-1 (hPON1)

Abstract

hPON1 is an enzyme from the group of A-esterases which is capable of hydrolyzing the active metabolite paraoxon of parathion, an organic phosphorus insecticide. It is an important liver enzyme that plays a protective role against the hydrolysis of HDL-induced organophosphate agents and nerve gases, oxidation of LDL, formation of lipid peroxides and bacterial endotoxins. The fact that oxidation of LDL constitutes the initial stage of the atherosclerosis process reveals the importance of the antioxidant properties of the enzyme. In this study, human serum PON1 was purified using three simple biochemical purification techniques. Furthermore, the in vitro effects of some antibacterial and antiviral drugs on human serum PON1 enzyme activity were examined. IC50 values were determined.