Abstract
Objective To observe the inhibition effect of the hypoxia inducible factor-lα （HIF-lα） specific siRNA on the expression of vascular endothelial growth factor （VEGF） mRNA in retinal tissues in diabetic rat. Methods This is a randomized controlled study. HIF-la specific siRNA recombinant plasmid was built in pSilencer2. 1-U6neo vector. Fifty-four healthy Sprague Dawley （SD） rats were divided into control group （15 rats） and experimental group （39 rats）. The experimental rats were induced with streptozotocin injection for diabetic retinopathy model, and then randomly divided into diabetic retinopathy （DR） group （15 rats）, vector group （12 rats） and gene therapy group （12 rats）. LipofectamineTM2000 mixed with pSilencer2. 1-U6neo plasmid or HiF-la siRNA plasmid were injected into the vitreous in the vector group and gene therapy group respectively. Nothing was transfected into DR and control group. The expression of VEGF mRNA in retinas was measured by real-time RT-PCR. The inhibition efficiency of VEGFmRNA was calculated at 24, 48, 72 hours and 1 week after injection respectively. Significant differences between groups were evaluated by one-way analysis and LSD-t analysis. Results HIF-la siRNA recombinant plasmid was confirmed by enzyme digestion and sequence analysis. Real-time RT-PCR revealedthat the expression of VEGFmRNA was faint in the control group, increased obviously in the DR and vector group, decreased in the gene therapy group. There was no statistically significant between DR and vector group （t=0. 669,0.142,0.151,0. 025;P=0; 514,0. 889,0. 882,0. 980）. The expression of VEGFmRNAin the gene therapy group were obviously decreased compared with DR and vector group （t= 8. 768, 13. 695, 11.285, 8. 253; P=0.000）. The inhibition efficiency of VEGFmRNA was 32.76%, 43.60%, 47.70%, 50.86% at 24, 48, 72 hours and 1 week after injection. Conclusions The expression of VEGFmRNA can be efficiently inhibited by HIF-Iα siRNA recombinant plasmid. Key words: Diabetic retinopathy/pathophysiology; Retinal neovascularization; Hypoxia-induciblefactor 1; alpha subunit; Vascular endothelial growth factors; RNA Interference; Diabetes mellitus,experimental
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
