Expression of Twist protein, vascular endothelial growth factor mRNA and protein and their relation to biological characteristics of laryngeal squamous cell carcinom

Abstract

Objective To observe the expression of Twist protein, vascular endothelial growth factor (VEGF) mRNA and protein in laryngeal squamous cell carcinoma (LSCC) and their relation to clinical pathological characteristics, and aslo debate their effect and clinical application in carcinogenesis, developing of LSCC. Method Molecular hybridization in situ was carried to detect VEGF mRNA, and immunohistochemistry S-P method was carried to detect the expression of Twist protein and VEGF protein in 80 cases of LSCC, 30 cases of paraneoplastic tissue. Results The expression of Twist protein, VEGF mRNA and protein was related to TNM stage, T stage, thyroid cartilage involvement, lymph node metastasis and distance metastasis (P ＜ 0.05 or ＜ 0.01 ). The expression of Twist protein and VEGF protein was related to differentiated degree (P ＜ 0.01 or ＜ 0.05), however, the expression of VEGF mRNA was not related to differentiated degree (P ＞ 0.05 ). The expression of Twist protein was positively related to the expression of VEGF protein in LSCC (r =0.334, P ＜ 0.01 ) ; and the expression of VEGF mRNA was positive correlation to the expression of VEGF protein (r =0.484,P ＜ 0.01 ). Conclusions Overexpression of Twist protein and VEGF mRNA and protein might play a joint action in the carcinogenesis, development of LSCC. Combined detection of Twist protein and VEGF mRNA and protein has vital significance in predicting the invasion and metastasis of LSCC, which might be an index to judge the biological behavior of LSCC. Key words: Twist transcription factor; Vascular endothelial growth factor; Laryngeal neoplasms; Immunohistochemistry