Inhibition activity of α-glucosidase enzyme, phenolic content and toxicity of Mara (Macaranga tanarius (L.) Mull. Arg) Leaf

Abstract

Previous studies have shown that mara leaves (Macaranga tanarius (L) Mull. Arg) can be used as medicinal plants because they have antibacterial and anti-inflammatory activity. Compounds containing flavonoids, tannins, saponins. But there is still little research on mara leaves as inhibition α-glucosidase. The purpose of this study was to determine the antidiabetic activity by the α-glucosidase method and its toxicity by the BSLT method and the results of fractionation. This test can be done by looking at the IC50 values obtained from linear regression analysis. Whereas the acute toxicity test uses BSLT (method of Shrimp Lethality Test). Using concentrations of 1000 ppm, 500 ppm, 100 ppm and 10 ppm. At each concentration using 10 larvae Artemia salina Leachand analyzed mortality. LC50 values were obtained from linear regression of mortality percentages and log doses. The results of this study prove the ethyl acetate fraction of Macaranga tanarius (L) Mull. Arg leaves has a high value inhibition activity that is 0.74 μg/mL and is non-toxic so it can be considered as inhibition α-glucosidase.