Influenza virus増殖に及ぼす孵化鶏卵漿尿液のpH並びにInhibitorの影響

Abstract

Influences of pH and inhibitor of chorioallantoic fluid on the multiplication and infectivity of influenza virus were observed in detail with indivisual eggs infected. PR8 strain of influenza A virus was employed throughout this study. When incubated at 37°C, in control eggs, the pH of chorioallantoic fluid falled rapidly since about the 14th incubation day. The values of pH of the chorioallantoic fluid corresponding to the harvest on the first time were higher than those corresponding to that on the second time or more of the successive harvestion from day, to day when observation was made with eggs in same incubation day. And the difference between the two values was about 0.5.While, in the infected eggs with the virus, the distinct descending of the pH of the fluid as observed in the control eggs was not recognizable. When the virus was infected to the eggs the pH of whose chorioallantoic fluid was in rapidly descending stage, the characteristic re-ascending curve of pH was recognized. The pH value under 6.0 seemed to act as inhibiting the multiplication of the virus; however, even such low pH value did not demonstrate a absolute negative significance for the infection of the virus.The inhibitors from chorioallantoic fluid, egg white and egg yolk had no influences on the multiplication of the virus. It appeared likly that the inhibitors from chorioallantoic fluid and egg white were closely analogous to mucoprotein from their attitude to the action of RDE and heating. To the contrary, the inhibitor from egg yolk was considered to be derived from the other substance.Both the wave height of polarographic catalytic protein wave of chorioallantoic fluid due to the sulfosalicylic acid filtrate test and the inhibiting titer of the same fluid for the hemagglutination of the virus were raised as the incubation day was progressed, and a definite relationship was observable between them. The inhibitor of mucoprotein nature was proved to exist also in the infected fluid and in this connection no significant difference was recognized between normal and infected fluid. The eggs on the 17th incubation day were able to be rendered to show as high a hemagglutinating titer as that on the 12th incubation day to the inoculation of the virus, when a certain volume of physiological saline was added to the chorioallantoic cavity and thus the volume of the fluid was increased.