Polarograph法の細菌学的応用

Abstract

The author examined the polarographic effect of proriferation of influenza virus on catalytic protein waves exhibited by the chorioallantoic fluid of developing chick embryo and obtained the fllowing results.Catalytic protein waves of the chorioallantoic fluid discerned in various conditions can be classified into five types. They were designated as A, B, C, D and E waves respectively. Generally speaking, in normal eggs, the waves appeared in the order of A waves to E in sequence as the incubation days prgressed. In the inoculated eggs with the virus, the process was delayed. with wave height, in the normal eggs, it rose until the sixteenth day, as the incubation days progressed. While in the inoculated eggs the rise was not distinct, and the significant difference was recognized between the two.A, B and C waves presented characteristic single catalytic waves and was analogous to catalytic cystine waves. E wave was observed as double catalytic waves and was shown to have the similar nature as serum protein waves. A possibility that the above facts can be utilized to know the existence of multiplication of viruses in the developing chick embryo, whether they are already known or quite new, was discussed.