Abstract

We previously demonstrated the anti‐influenza activity of Citrullus lanatus var. citroides (wild watermelon, WWM); however, the active ingredient was unknown. Here, we performed metabolomic analysis to evaluate the ingredients of WWM associated with antiviral activity. Many low‐molecular weight compounds were identified, with flavonoids accounting for 35% of all the compounds in WWM juice. Prenylated flavonoids accounted for 30% of the flavonoids. Among the measurable components of phytoestrogens in WWM juice, 8‐prenylnaringenin showed the highest antiviral activity. We synthesized 8‐prenylnaringenin and used liquid chromatography–mass spectrometry to quantitate the active ingredient in WWM. The antiviral activities of 8‐prenylnaringenin were observed against H1N1 and H3N2 influenza A subtypes and influenza B viruses. Moreover, 8‐prenylnaringenin was found to inhibit virus adsorption and late‐stage virus replication, suggesting that the mechanisms of action of 8‐prenylnaringenin may differ from those of amantadine and oseltamivir. We confirmed that 8‐prenylnaringenin strongly inhibited the viral entry of all the influenza virus strains that were examined, including those resistant to the anti‐influenza drugs oseltamivir and amantadine. This result indicates that 8‐prenylnaringenin may activate the host cell's defense mechanisms, rather than directly acting on the influenza virus. Since 8‐prenylnaringenin did not inhibit late‐stage virus replication of oseltamivir‐resistant strains, 8‐prenylnaringenin may interact directly with viral neuraminidase. These results are the first report on the anti‐influenza virus activity of 8‐prenylnaringenin. Our results highlight the potential of WWM and phytoestrogens to develop effective prophylactic and therapeutic approaches to the influenza virus.

Highlights

  • Influenza is an acute respiratory infection caused by the influenza virus (IFV), which belongs to the family Orthomyxoviridae and is prevalent worldwide

  • We focused on prenylated naringenins, such as 8-PN (Fig. 1a), which was detected by liquid chromatography-mass spectrometry measurement (QQQ) at approximately 1.5 ng/g in the wild watermelon (WWM) juice

  • It was hypothesized that the anti-influenza effect of flavonoids might stem from their ability to coordinate metal ions, as documented by various quercetin-metal ion complexes reported in the literature [28, 29]

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Summary

Introduction

Influenza is an acute respiratory infection caused by the influenza virus (IFV), which belongs to the family Orthomyxoviridae and is prevalent worldwide. Types A, B, and C IFV can infect humans with types A and B influenza, causing seasonal epidemics every year and sometimes causing severe complications, such as pneumonia and encephalitis [1]. Vaccines and antiviral drugs are used to prevent and treat IFV infection, respectively. These vaccines fail to induce a stable preventive effect [2]. The emergence of IFV strains resistant to amantadine and oseltamivir has become a serious problem in recent years [3, 4]. A novel approach to protect against IFV infection is needed

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