Abstract
Objective To investigate influences of pretreating with lipopolysaccharide (LPS) the infant epilepsy rats on the gene expression of Toll-like receptor 4/high mobility group protein 1 (TLR4/HMGB1) gene and astrocytes proliferation in the hippocampus in the chronic phase. Methods The rats on postnatal 21 day (21d) were randomly divided into control group, model Ⅰ group and model Ⅱ group.The model groups were induced into epilepsy model by using kainic acid (KA), and the model Ⅱ group was pretreated with intraperitoneal injection of LPS 2 hours before KA application.Behaviors of infant rats with epilepsy seizures were observed and given scores according to Racine score, and the gene expressions of TLR4 and HMGB1 at 3 h and 24 h after status epilepticus (SE) were detected by using fluorescence quantitative PCR, and astrocyte proliferation on 21 day after SE were detected by using immunohistochemistry, and the gial fibrillary acidic protein (GFAP) was detected. Results The TLR4 gene expressions at 3 h after SE in hippocampus of model group Ⅰ and model group Ⅱ were significantly higher than that of control group (t=4.806, 4.954, all P 0.05). The HMGB1 gene expressions at 3 h after SE in hippocampus of model group Ⅰ and model group Ⅱ were significantly higher than that of control group (t=3.626, 5.255, all P 0.05). The HMGB1 gene expressions at 24 h after SE in hippocampus of model group Ⅰ and model group Ⅱ were significantly higher than that of control group (t=4.046, 2.836, all P 0.05). The GFAP positive cells on 21 day after SE in hippocampus of control group were few, and were more significant in model group Ⅰ and model groupⅡ. Conclusions The expression of TLR4/HMGB1 gene in the hippocampus of infant epilepsy rats increased in acute phase, and LPS pretreatment can increase the expression of TLR4 gene in hippocampus of infant rats with epilepsy and the proliferation of astrocytes in the chronic phase. Key words: Lipopolysaccharide; Kainic acid; Epilepsy; Infant rat; High mobility group protein 1; Toll-like receptor 4
Published Version
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