Abstract
Objective To investigate the immunopotentiating effects of cultivated Cistanche deserticola (C.deserticola) crude polysaccharides (CCDCP) as an adjuvant on the model antigen ovalbumin (OVA). Methods Low, medium and high doses of CCDCP in combination with OVA were intramuscularly injected twice into ICR mice at an interval of two weeks, respectively. Aluminum adjuvant was used to set up positive control group. Levels of IgG, IgG1 and IgG2a antibodies were detected by ELISA. Splenocyte proliferation was detected by MTT assay. Growth conditions of the immunized mice were observed. Results IgG level was significantly increased in the high dose group 7 days after the first immunization (P 0.05). Conclusion CCDCP as an adjuvant of OVA protein vaccine can enhance Th1 and Th2 immune responses, especially the early antibody production and Th1 immune response. These results will provide some information for further studies of CCDCP as a vaccine adjuvant. Key words: Cultivated C.deserticola; Crude polysaccharides; OVA; Intramuscular immunization
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