Abstract
Introduction: Burns are one of the most common household and industrial injuries. There are evidences which demonstrate the therapeutic properties of honey and Aloe vera. We evaluated the topical influences of this material and nano zinc combination on healing the wounds caused by third-degree burns. Materials and methods: 32 balb/c mice divided into a control group (without treatment), group 1 (treated with Aloe vera and nano zinc), group 2 (treated with Aloe vera, honey and nanoz inc) and group 3 (treated with honey and nano zinc). The third-degree burn was created on the back of balb/c mice with general anesthesia observing sterile conditions. Local treatment of burn was conducted once a week during 6 weeks and after the end of treatment, were anesthetized by ether and then killed. After fixation, the practical steps of general histology technique were performed on it. The samples stained with hematoxylin–eosin and they observed with a microscope. Results: We found full tightening of the burn wound and less scar in the group treated with nano zinc and honey compared to control group and other groups. In histological studies, a significant increase was found in the overall thickness of the skin, keratinocyte layer, the epidermis and hypodermis, number and diameter of the hair follicles in a third group versus other groups. Conclusion: The results showed the organic honey and nano zinc combination accelerate the healing process of burn wound in male balb/c mice. While adding Aloe vera to this composition doesn't have an effect on wound healing.
Highlights
Burns are one of the most common household and industrial injuries
Macroscopic findings: Macroscopic images are taken from wound place in experimental groups after passing treatment period confirm complete tightening of the wound in the third group treated with Honey and nano zinc versus the other group
Topical treatment in a group treated with the combination of Honey and nano zinc left less scar compared to other groups
Summary
Burns are one of the most common household and industrial injuries. There are evidences which demonstrate the therapeutic properties of honey and Aloe vera. We evaluated the topical influences of this material and nano zinc combination on healing the wounds caused by third-degree burns. Results: We found full tightening of the burn wound and less scar in the group treated with nano zinc and honey compared to control group and other groups. Conclusion: The results showed the organic honey and nano zinc combination accelerate the healing process of burn wound in male balb/c mice. While adding Aloe vera to this composition doesn't have an effect on wound healing. Aloe vera gel increases the wound collagen rate and changes the composition of collagen as well as collagen crosslinking. As a result, it accelerates wound healing. Using Honey to treat wound was very common in medicine of countries such as Greece, Egypt and
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