Influences of APE1 gene silence on biological behavior of human hepatoma carcinoma cell line MHCC97-H

Abstract

Objective To investigate the influences of apurinic/apyrimidinic endonuclease ( APE1 ) gene silence on malignant behaviors in vitro-in vivo of human hepatoma carcinoma cell line MHCC97-H with high metastasis potential. Methods Three groups were set up: LV-APE1-shRNA transfected group, LV-APE1-NC empty vector transfected group and non-transfected group (control group). The ability of proliferation, adhesion, motility and invasion in vitrowas measured by using MTT assay and Transwell chamber. An animal model of the human hepatoma cancer xenograft was established. The intact subcutaneous tumor tissues were obtained and their volumes and weight were measured. Tumor growth inhibition ratio was calculated. Reverse transcription-polymerase chain reaction (RT-PCR) assay and Western blotting were performed to detect the expression level of APE1. Results The ability of proliferation, adhesion,motility and invasion in LV-APE1-shRNA transfected group was lower as compared with other two groups (P ＜0. 01 ). In LV-APE1-shRNA transfected group, the subcutaneous tumors grew slowly, the volume (0. 467 ±0. 052) cm3 and weight (0. 267 ±0. 082) g were reduced (P ＜0. 01 ). The tumor growth inhibition ratio was 75. 881%. The expression levels of APE1 mRNA was decreased by 90. 584% and those of protein by 72. 439%. Conclusion APE1 gene silence of MHCC97-H can inhibit the ability of proliferation and invasion in vitro-in vivo. APE1 gene has effect on the tumor malignant behaviors of hepatoma carcinoma cells. Key words: Carcinoma,hepatocellular; APE1; Nude mice; Invasion