Abstract
Objective To filtrate high expression of homeo box A13 (HOXA13) in hepatoma carcinoma cells and investigate the effects of HOXA13 (liver-intestine cadherin) RNA interference (RNAi) in high hepatoma expression carcinoma cells on biological effects. Methods The expression levels of HOXA13 mRNA and protein in hepatoma carcinoma cells were detected by reverse transcriptase-polymerase chain reaction (RT-PCR) and Western blotting to filtrate high expression of HOXA13 in hepatoma carcinoma cells, with Lipofectamine and plasmid to transfect high expression of HOXA13 in hepatoma carcinoma cells. The expression levels of HOXA13 mRNA and protein in cells transfected with HOXA13-RNAi were detected by RT-PCR and Western blotting, respectively. The proliferative activity of hepatoma carcinoma cells was measured by cell counting kit-8 (CCK-8) method. The migration and invasive capabilities of hepatoma carcinoma cells were examined by scratch-wound migration assay and Transwell invasion assay respectively. Results The results of RT-PCR and Western blotting revealed that the expression levels of HOXA13 mRNA and protein in MHCC-97H cells (14.010±0.431, and 0.702±0.062) were signifeicantly higher than in HepG2, Huh-7, SMMC-7721 cells (12.292±0.821, 0.653±0.087; 11.750±1.271, 0.527±0.069; 11.242±2.031, 0.420±0.075, P<0.05). The results of RT-PCR and Western blotting revealed that the expression levels of HOXA13 mRNA and protein in MHCC-97H cells transfected with HOXA13-RNAi (3.215±0.022, and 0.117±0.016) were lower than in control group (9.426±1.006, 0.364±0.025; 10.517±1.412, 0.426±0.047, P<0.05). The proliferation ability in HOXA13-RNAi group (0.368±0.115) was significantly lower than in the negative control group and blank control group (0.688±0.352, and 0.684±0.322, P<0.01). The proliferation of HOXA13-RNAi MHCC-97H cells was decreased significantly due to HOXA13 knockdown (P < 0.001). The invasive and migration capabilities of MHCC-97H cells transfected with HOXA13-RNAi [(47.302±5.410) cells] were also significantly reduced as compared with the negative control group and blank control group [(82.221±11.102) cells, and (84.322±12.520) cells, P<0.01]. Conclusion Knockdown of HOXA13 may play an important role in the proliferation, invasion and migration of human hepatoma carcinoma cells and it can promote the oncogenesis and progression of human hepatoma carcinoma. Key words: Homeo box A13; RNA interference; Carcinoma, hepatocellular
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