Abstract
In this paper, the influence of negatively charged active fluorescent dextran on electrofusion of barley protoplasts was investigated. We discovered that this biopolymer inhibits the electrofusion process. The adsorption of fluorescent dextran on membranes was determined by observing their fluorescence microphotograph in protoplast suspension. A green fluorescent ring appeared around the membrane. The adsorption phenomena were also investigated by measuring the influence of additives on electrophoresis of protoplasts. It was detected that the membrane of barley protoplasts had a negative charge. After being stored in solution containing fluorescent dextran, the protoplasts demonstrated increased relative mobility due to the increase of negative charge on membranes by adsorption. The mobility decreased when the protoplasts were pretreated in the solution containing positively charged substances Bardac 2080. The increase of the membrane's negative charge by adsorption F-DX enhances the repulsion between protoplasts, and therefore decreases the electrofusion yield.
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