INFLUENCE OF TRIMETAZIDINE METABOLIC THERAPY ON CONNECTIVE TISSUE METABOLISM IN EXPERIMENTAL DIFFUSE ISCHEMIC NECROTIC CARDIOSCLEROSIS IN RATS WITH DIFFERENT RATES OF HYPOXIA RESISTANCE

Abstract

<p>Background. The change in metabolism of the connective tissue elements of heart is the central chain in<br />pathogenesis of diffuse ischemic necrotic cardiosclerosis (DINC), which occurs after repeated epinephrine injury<br />of myocardial tissues.<br />Objective. This study proves that trimetazidine (TM) metabolic therapy has a protective effect on the<br />development of DINC in rats with different rates of hypoxia resistance.<br />Methods. Male white rats were divided into three groups due to the different rates of hypoxia resistance by<br />means of the method of hypobaric hypoxia: rats with low, middle and high rates of hypoxia resistance. Each<br />group was divided into equal subgroups: a control group, a DINC group (injections of epinephrine hydrotartrate<br />(0,5 mg/kg of body weight) and calcium gluconate (5 mg/kg of body weight) two times), a control group administrated<br />with trimetazidine dihydrochloride (10 mg/kg of body weight), a DINC group treated with TM every day<br />(10 mg/kg of body weight) for all period of observation. Concentration of protein-bound oxyproline in blood<br />serum was evaluated on the 7th, 14th and 30th days after the pathology simulation. Histological examination of<br />Masson trichrome staining of myocardium was performed on the 30th days after the pathology simulation.<br />Results. DINC increased the concentration of protein-bound oxyproline in blood serum on the 7th, 14th and<br />30th days after the pathology simulation, and followed by metabolic imbalances in diffuse connective tissue elements,<br />which are rich in collagens. DINC+TM increased the concentration of protein-bound oxyproline in blood<br />serum less intensively.<br />Conclusions. The intensity of metabolic imbalances in diffuse connective tissue elements is the highest in<br />the low resistant animals to hypoxia. Those results are confirmed by histological examination of the myocardium<br />of rats with different resistance to hypoxia. Fibrotic regions in myocardium are rich in collagens. It has been<br />revealed that the most pronounced therapeutic effect of TM is observed in animals with low resistance to hypoxia,<br />slight – in animals with medium resistance to hypoxia, and the lowest – in animals with high resistance to<br />hypoxia.<br />KEY WORDS: hypoxia, heart, diffuse cardiosclerosis, trimetazidine, oxyproline.</p>