Influence of Tributyltin on in Vitro Activation of Oyster Toadfish Macrophages

Abstract

Abstract The effects of tributyltin (TBT), phorbol myristate acetate (PMA), and calcium ionophore (A23187) on the chemiluminescent response of peritoneal macrophages from oyster toadfish Opsanus tau were evaluated in vitro. The PMA-stimulated chemiluminescent response was increased by exposure to 50 μgTBT/L but was depressed to baseline values by 500 μg/L. With A23187-stimulated chemiluminescence, the response was increased by exposure to 5 and 50 μgTBT/L and was depressed by 500 μg/L. The chemiluminescent response stimulated by synergistic doses of PMA and A23187 was increased by exposure to 50 μgTBT/L but was depressed by 500 μg/L. Chemiluminescence, enhanced by PMA and A23187 after TBT exposure, returned to baseline values after extracellular calcium was removed. In the absence of PMA or A23187 stimulation, 50 μgTBT/L stimulated a significant chemiluminescent response that also was inhibited by chelation of extracellular calcium. Uptake of calcium-45 was greatly increased in the presence of 50 μgTBT/L ...