Abstract
The authors studied the effect of treatment with bacteria Bacillus subtilis Cohn (strains 26D) and B. thuringiensis Berliner (strain B-6066) on the hydrogen peroxide (H2O2) content, the activity of hydrolytic enzymes and their protein inhibitors in potato plants (Solanum tuberosum L.) in connection with development of resistance to the late blight pathogen - oomycete Phytophthora infestans Mont. de Bary. Studies were carried out on potato plants of the susceptible Early Rose potato cultivar that were treated with a suspension of B. subtilis and B. thuringiensis bacteria (108 cells/ml) and infected with P. infestans (107 spores/ml). A decrease in the degree of leaf damage by oomycete was revealed under the influence of the genus Bacillus bacteria, depending on the strain. The increase in potato resistance to P. infestans infection was mediated by the stimulating effect of the B. subtilis 26D and the B. thuringiensis B-6066 bacteria on the concentration of H2O2, the modulating effect on the activity of hydrolytic enzymes and the enhancement of the transcriptional activity of protease and amylase inhibitor genes in plant tissues. Differences in the degree of activation of the transcriptional activity of hydrolase inhibitor genes by the B. subtilis 26D and the B. thuringiensis B-6066 bacteria were revealed, which suggests differential ways of forming the potato resistance to P. infestans under their influence.
Highlights
One of the ways of increase of plant resistance to pathogenic organisms and adverse environmental factors by ecologically friendly methods is the scientifically substantiated use of non-pathogenic rhizospheric bacteria (PGRP – plant growth promoting rhizobacteria)
Plants were grown on a light site for 15 days, inoculated with a suspension culture of B. thuringiensis B-6066 bacteria, obtained from the State Research Institute of Genetics and Selection of Industrial Microorganisms, and B. subtilis 26D bacteria from the commercial biological product “Fitosporin-M” (“Bashinkom”, Russia). 5 days after bacteria treatment, the plants were infected with a spore suspension (107 spores/ml) of P. infestans – the late blight pathogen
We used the culture of the oomycete Phytophthora infestans (Mont.) de Bary from the collection of the Institute of Biochemistry and Genetics of the Ufa Federal Research Centre of the Russian Academy of Sciences
Summary
One of the ways of increase of plant resistance to pathogenic organisms and adverse environmental factors by ecologically friendly methods is the scientifically substantiated use of non-pathogenic rhizospheric bacteria (PGRP – plant growth promoting rhizobacteria). An important feature of biological products based on living microorganisms is the non-specific activation of plant defense mechanisms [4]
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