Abstract
The following field experiences were performed on the Chernozem southern carbonate: 1.Control, 2. Sulphoammophos – 150 kg/ha, 3. Sulphoammophos – 250 kg/ha, 4.Phosphogypsum – 3 t/ha, 5.Phosphogypsum – 6 t/ha, 6.Phosphogypsum – 12 t/ha, 7. Phosphogypsum – 3 t/ha ammophos –70 kg/ha ammonium nitrate, 100 kg/ha 8. Phosphogypsum – 6 t/ha ammophos -70 kg/ha ammonium nitrate, 100 kg/ha 9. Phosphogypsum – 12 t/ha ammophos–70 kg/ha ammonium nitrate, 100 kg/ha. 10. ammophos-70 kg/ha + ammonium nitrate 100 kg/ha. The determination of the number of microorganisms produced in the phase of milky-wax ripeness of winter wheat on the selective nutrient medium: meat-peptone agar – number of ammonifiers; on starch and ammonia agar – microorganisms that assimilate mineral forms of nitrogen on the Hutchinson medium – calculatorsreal the number of microorganisms on the Czapek-Dox medium – the number of micromycetes on the Ashby medium – the number of aerobic nitrogen-fixing bacteria of the genus Azotobacter. Introduction phosphogypsum, sulphoammophos, ammophos and ammonium nitrate contributes to the increase in the numbers of various physiological groups of microorganisms. The highest values of the studied indicators in comparison with the control was achieved through the joint application of phosphogypsum – 12 t/ha ammophos-70kg/ha and ammonium nitrate-100kg/ha: ammonifiers on 123.4 million CFU/g (or 2.5 times); nitrifiers at 138 million CFU/g (3.3 times), microscopic fungi on 90,0 thousand CFU/g (1.7 times); cellulose-fermenting microorganisms on 250.6 thousand CFU/g (more than 2 times); aerobic nitrogen-fixing bacteria of the genus Azotobacter by 30.7 thousand CFU/g (1.5 times). Thus, the most responsive to the introduction of phosphogypsum and fertilizers microorganisms that convert mineral and organic nitrogen compounds.
Highlights
The microbial population plays a special role in formation of soil fertility and productivity of agricultural land
The determination of the number of microorganisms produced in the phase of milky-wax ripeness of winter wheat on the selective nutrient medium: meat-peptone agar – the number of ammonifiers; on starch and ammonia agar – microorganisms that assimilate mineral forms of nitrogen on the Hutchinson medium – the number of calculatorsreal microorganisms on the Czapek-Dox medium – the number of micromycetes; on the Ashby medium – the number of aerobic nitrogenfixing bacteria of the genus Azotobacter
The result of the research showed that the introduction of phosphogypsum and fertilizers had an impact on the number of microorganisms of various physiological groups
Summary
The microbial population plays a special role in formation of soil fertility and productivity of agricultural land. They determine the rate of biological weathering of mineral bases in soils, decomposition of organic mortmass and transform unavailable forms of nutrients available to plants [1, 2]. As a result of agricultural use of the soil, they are constantly depleted due to the removal of nutrients and their alienation along with the harvest [4, 5]. This applies to mobile sulfur, which determines the quality of the yield. According to many scientists [9], the elimination of sulfur deficiency in the soil contributes to obtaining a higher yield of better quality
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