Abstract
The partition of proteins in a poly(ethylene glycol) (PEG)–potassium phosphate aqueous two-phase system has been studied in systems which were produced by manipulation of the equilibrium temperature of a monophasic mixture composition close to the critical point. Precise control of the composition enabled the formation of biphasic systems lying very close to the critical point. A high-performance liquid chromatographic method for analysis of the phase compositions is presented. The generally applicability of the method to the partition of proteins and other particles is discussed.
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Schedule a callMore From: Journal of Chromatography B: Biomedical Sciences and Applications
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