Abstract
This research investigated the influence of soil microbiota on Escherichia coli O157:H7 survival in soil rinse and artificial soil. Additionally, the influence of selected soil bacteria on E. coli O157:H7 in soil environments was determined. Escherichia coli O157:H7 counts (log CFU per ml or g-1 ) were determined by spread plating: (i) artificial soil amended with soil rinse (filter-sterilized and unfiltered) at 30°C; (ii) unfiltered soil rinse (50ml) treated with cycloheximide (200μgml-1 ), vancomycin (40μgml-1 ), heat (80°C, 15min) and no treatment (control) for 7days at 30°C and (iii) filtered soil rinse with selected soil bacterial isolates over 7days. There was a significant difference (P=0·027) in E.coli O157:H7 counts after 35days between artificial soils amended with filtered (4·45±0·29) and non-filtered (1·83±0·33) soil rinse. There were significant differences (P<0·05) in E.coli O157:H7 counts after 3days of incubation between soil rinse treatments (heat (7·04±0·03), cycloheximide (6·94±0·05), vancomycin (4·26±0·98) and control (5·00±0·93)). Lastly, a significant difference (P<0·05) in E.coli O157:H7 counts was observed after 3days of incubation at 30°C in filtered soil rinse when incubated with Paenibacillus alvei versus other soil bacterial isolates evaluated. Soil microbiota isolated from Florida sandy soil influenced E.coli O157:H7 survival. Specifically, P. alvei reduced E.coli O157:H7 by over 3 log CFU per ml after 3days of incubation at 30°C in filtered soil rinse. This research identified soil bacterial isolates that may reduce E. coli O157:H7 in the soil environment and be used in future biocontrol applications.
Published Version
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