Influence of rhizobacterial volatiles on the root system architecture and the production and allocation of biomass in the model grass Brachypodium distachyon (L.) P. Beauv.

Pierre Delaplace,Caroline Baudson,Magdalena Mendaluk-Saunier De Cazenave,Yves Brostaux,Stijn Spaepen,Benjamin M Delory,Patrick Du Jardin,Sébastien Varin

doi:10.1186/s12870-015-0585-3

Abstract

BackgroundPlant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems. The effects on their host plants are diverse and include volatile-mediated growth enhancement. This study sought to assess the effects of bacterial volatiles on the biomass production and root system architecture of the model grass Brachypodium distachyon (L.) Beauv.ResultsAn in vitro experiment allowing plant-bacteria interaction throughout the gaseous phase without any physical contact was used to screen 19 bacterial strains for their growth-promotion ability over a 10-day co-cultivation period. Five groups of bacteria were defined and characterised based on their combined influence on biomass production and root system architecture. The observed effects ranged from unchanged to greatly increased biomass production coupled with increased root length and branching. Primary root length was increased only by the volatile compounds emitted by Enterobacter cloacae JM22 and Bacillus pumilus T4. Overall, the most significant results were obtained with Bacillus subtilis GB03, which induced an 81 % increase in total biomass, as well as enhancing total root length, total secondary root length and total adventitious root length by 88.5, 201.5 and 474.5 %, respectively.ConclusionsThis study is the first report on bacterial volatile-mediated growth promotion of a grass plant. Contrasting modulations of biomass production coupled with changes in root system architecture were observed. Most of the strains that increased total plant biomass also modulated adventitious root growth. Under our screening conditions, total biomass production was strongly correlated with the length and branching of the root system components, except for primary root length. An analysis of the emission kinetics of the bacterial volatile compounds is being undertaken and should lead to the identification of the compounds responsible for the observed growth-promotion effects. Within the context of the inherent characteristics of our in vitro system, this paper identifies the next critical experimental steps and discusses them from both a fundamental and an applied perspective.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-015-0585-3) contains supplementary material, which is available to authorized users.

Highlights

Plant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems
300 candidate molecules have been identified to date, very few have been unequivocally identified as being responsible for the observed change in plant growth [8, 9], mainly because bacterial volatiles can act as individual compounds or in mixtures [13]
Characteristics of the in vitro co-cultivation system In order to expose B. distachyon Bd21 plantlets to bacterial volatile compounds and assess their effects on biomass production while measuring root system architecture (RSA) parameters, a near-vertical co-cultivation system was set up (Fig. 1)

Summary

Introduction

Plant growth-promoting rhizobacteria are increasingly being seen as a way of complementing conventional inputs in agricultural systems. The underlying mechanisms include; (1) root system architecture (RSA) modulation and increased shoot growth, mediated by indole-3-acetic acid, cytokinins, gibberellins, salicylic acid, ethylene and brassinosteroids; (2) phosphate solubilisation; (3) free nitrogen fixation; (4) suppression of harmful microorganisms; (5) induced systemic resistance; and (6) induced systemic tolerance of abiotic constraints [1,2,3,4, 6, 7] Among these interaction mechanisms, the emission of bacterial volatile organic compounds (VOCs) has been shown to promote plant growth [6] and VOCmediated plant growth modulation is widely considered to be an important mechanism [8]. 300 candidate molecules have been identified to date, very few have been unequivocally identified as being responsible for the observed change in plant growth [8, 9], mainly because bacterial volatiles can act as individual compounds or in mixtures [13]

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