Abstract
The effect of adding protein on the decomposition behavior of lignin in Japanese cedar under supercritical methanol conditions (270 °C/27 MPa) was studied. The Klason method was used to detect the lignin content in the insoluble residue following to a 30 min treatment. Adding either an animal (bovine serum albumin) or plant (soy) protein enhanced delignification from 50 to 65% of the lignin-based wt %. This result was attributed to enhanced lignin depolymerization owing to inhibited lignin recondensation and/or the suppressed formation of polysaccharide-derived char via reactions between the protein and polysaccharides. Although the solubilization of lignin was promoted and the yield of lignin-derived low-molecular-weight compounds increased, the selectivity of major monomers such as coniferyl alcohol (CA) and γ-methylated CA decreased. The addition of proteins has a substantial impact on the decomposition behavior of cell wall components under supercritical methanol conditions. This information provides insights into the use of protein-rich lignocelluloses.
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