Abstract
The multiple molecular forms (isoenzymes) of glucose–6–phosphate dehydrogenase and lactate dehydrogenase at various states of differentiation in the functional rat mammary gland were examined and the isoenzyme changes were related to DNA, RNA and protein levels. Three isoenzymes of glucose–6–phosphate dehydrogenase were demonstrated. In terms of activity, one species (G6PD–3) remained relatively unchanged during the periods of study; one species (G6PD–2) increased in both pregnancy and lactation, apparently correlated with increased estrogen levels; and a third species (G6PD–1) only appeared in late pregnancy, and increased markedly during lactation becoming the predominant species. Involution brought about a return of the glucose–6–phosphate dehydrogenase isoenzyme pattern to that seen in the quiescent state. The activities of the muscle—type lactate dehydrogenase isoenzymes (LDH–5, LDH–4) increased substantially in activity during lactation, while LDH–3, LDH–2 and LDH–1 showed little or no change. These...
Published Version
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