Abstract
<p>Mycobacterium tuberculosis is a major infection agent of tuberculosis that is controlled by the response of cell-mediated immunity. It is macrophages and cytolytic T lymphocytes. Activated macrophages will produce free radicals. Excessive free radicals cause tissue damage. Polysaccharide krestin contains β-glucan. It is a scavenger of free radicals. This research aimed to identify the influence of polysaccharide krestin from C. versicolor on nitrite and malondialdehyde concentrations of mice serum exposed by M. tuberculosis. Nitrite concentration was determined by nitrite assay. Malondialdehyde concentration was determined by TBARS assay. The result showed that adding polysaccharide krestin before exposure (P1) and adding polysaccharide krestin before-after exposure (P3) had the best potential to decrease nitrite concentration. Nitrite concentrations of P1 and P3 were 1.364 ± 0.523 M and 1.456 ± 0.712 M respectively. Meanwhile, P1 group and adding polysaccharide krestin after exposure (P2) had the best potential to decrease malondialdehyde concentration. Malondialdehyde concentrations of P1 and P2 were 1125.86 ± 97.96 µM and 953.86 ± 328.16 µM respectively. Their nitrite and malondialdehyde concentrations decreased, compared to K and K- groups. The research conclusion was that adding polysaccharide krestin before exposure could decrease both nitrite and malondialdehyde concentrations.</p><p><strong>How to Cite</strong></p><p>Wahyuningsih, S., Pramudya, M., &amp; Sugiharto, S. (2016). Influence of Polysaccharide Krestin from Coriolus versicolor Extract on Nitrite and Malondialdehyde Concencentrations of Mus musculus Serum Exposed by Mycobacterium tuberculosis. <em>Biosaintifika: Journal of Biology &amp; Biology Education</em>, 8(1), 12-17.</p>
Highlights
METHODSTuberculosis is infectious disease caused by Mycobacterium tuberculosis
Individual who is infected by infectious disease, virulent, and pathogen will give immune responses, as both specific immune response and nonspecific immune response
The objective of this research was to know the influence of polysaccharide krestin from Coriolus versicolor extract on nitrite and malondialdehyde concentrations of mice serum exposed by Mycobacterium tuberculosis
Summary
Tuberculosis is infectious disease caused by Mycobacterium tuberculosis. In 2014, tuberculosis killed 1 .5 m i ll i o n p e o p le w o r ld w i d e. D u r i n g p h a g o c y t o s i s, r e a c t iv e o x y gen intermediates and nitric oxide in the form of peroxynitrite radicals has able to kill pathogen (Abbas et al ., 2 0 1 2 ). Polysaccharide krestin is extraction product of Coriolus versicolor. The objective of this research was to know the influence of polysaccharide krestin from Coriolus versicolor extract on nitrite and malondialdehyde concentrations of mice serum exposed by Mycobacterium tuberculosis. Coriolus versicolor extract method, adapted from Wahyuningsih (2014), Mycobacterium tuberculosis (0.25 Mc. Farland) was got from Balai Besar Laboratorium Kesehatan, Surabaya, East Java, Indonesia. Y is optical density value, and b is nitrite concentration. After 10 – 15 min incubation, optical density values were read at 540 nm.
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