Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa

Sri Puji Astuti Wahyuningsih,Nadyatul Ilma Indah Savira,Win Darmanto

doi:10.15294/biosaintifika.v8i3.6957

Sri Puji Astuti Wahyuningsih, Nadyatul Ilma Indah Savira + Show 1 more

Open Access

https://doi.org/10.15294/biosaintifika.v8i3.6957

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Abstract

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human. The wall cell of its bacteria contains lipopolysaccharide as virulency factors to protect it from human immunity. Lipopolysaccharide can inhibit phagocytosis in the body. Polysaccharide krestin (PSK) from Coriolus versicolor extract contains β-glucan that can increase phagocytic activity and capacity. This research aimed to identify the effect on polysaccharide krestin from C. versicolor extract on phagocytic activity and capacity of mice exposed by P. aeruginosa. The design of this research was experimental design. There were six treatment groups. The phagocytic activity and capacity were counted on slide smears of mice peritoneal fluid. The data was analyzed by using one way ANOVA. The results of the phagocytic activity and capacity showed that PSK was added before exposure (P1) or after exposure (P2) or before-after exposure (P3) had potential to increase phagocytic activity and capacity. The conclusion of the research was that adding polysaccharide krestin either before exposure or after exposure or both of them could increase phagocytic activity and capacity. The benefits of this research to development of science are expected to reduce human infection and to utilize natural ingredients as immunomodulator.How to CiteWahyuningsih, S. P. A., Savira, N. I. I, &amp; Darmanto, W. (2016). Effect on Polysaccharide Krestin from Coriolus versicolor Extract on Phagocytic Activity and Capacity of Mus musculus Exposed by Pseudomonas aeruginosa. Biosaintifika: Journal of Biology &amp; Biology Education, 8(3), 308-313.

Highlights

METHODSPseudomonas aeruginosa is an opportunistic bacterium that causes infections in human, such as urinary tract infections, respiratory tract infections, gastrointestinal infections and dermatitis (Hauser et al, 2005)
The objective of this research was to know the effect on Plysaccharide krestin from Coriolus versicolor extract on phagocytic activity and capacity of mice exposed by Pseudomonas aeruginosa
Polysaccharide krestin was obtained from Coriolus versicolor extract method, adapted from previous research, Pseudomonas aeruginosa

Summary

METHODS

Pseudomonas aeruginosa is an opportunistic bacterium that causes infections in human, such as urinary tract infections, respiratory tract infections, gastrointestinal infections and dermatitis (Hauser et al, 2005). Immunomodulator on P.aeruginosa infection will stimulate immune response by producing antibody, activating and proliferating T cell and macrophage, and activating NK cell that can eliminate infection cell and kill bacteria on its cell (Abbas et al, 2012). Beta-glucan can activate phagocyt cell, and has a role on antigen opsonization (Rifa’i, 2009). Polysaccharide krestin can use be curative to increase immune respon such of activating phagocyt (LeBlanc, 2006). The objective of this research was to know the effect on Plysaccharide krestin from Coriolus versicolor extract on phagocytic activity and capacity of mice exposed by Pseudomonas aeruginosa. Polysaccharide krestin was obtained from Coriolus versicolor extract method, adapted from previous research, Pseudomonas aeruginosa (0.25 Mc. Farland) was attained from Balai Besar Laboratorium Kesehatan, Surabaya, East Java, Indonesia. If there were significant differences, Duncan test (α=0.05) was taken to determine the best treatment among the treatment groups

AND DISCUSSION

Findings

CONCLUSION