Abstract
In Vitro shoot culture of Bulbophyllum dhaninivatii Seidenf. was conducted on semi-solid Vacin and Went (1949) medium supplemented with coconut water (50 100 and 150 ml/L), potato extract (25 and 50 g/L) and banana homogenate (25 and 50 g/L) for 12 weeks. The results found that the highest shoot number (6.92 shoots) could observe on the medium supplemented with 150 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate while the highest leaf and root number could obtain when cultured on the medium supplemented with 100 ml/L coconut water, 50 g/L potato extract and 50 g/L banana homogenate.
Highlights
Bulbophyllum is one of the largest genera in the Orchidaceae family
In vitro shoots of B.dhaninivatii were cultured on semi-solid VW medium supplemented with different concentrations of a combination between coconut water (CW), potato extract (PE) and banana homogenate (BH) for 12 weeks
The results indicated that morphological change of in vitro shoots could be noticed after the second week of culture
Summary
Bulbophyllum is one of the largest genera in the Orchidaceae family This genus comprised of about 3,000 species [1]. Was firstly found at Phu Miang Sub-District, Chatrakarn District, Phitsanulok Province Nowaday, this species is distributed in Phu Luang Wild Life Sanctuary, Loei Province and listed as an endemic species of Thailand [3]. In vitro culture and micropropagation Bulbophyllum species have been reported [11,12,13,14] and factors improving shoot multiplication in Bulbophyllum species, especially medium components, were studied [15,16,17]. Influence of other medium components, organic supplements, on shoot regeneration efficiency of Bulbophyllum species has only few performed in B. dhaninivatii. Our receiving results will be useful for conservation planning and used as a model for in vitro conservation of other Bulbophyllum species
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