INFLUENCE OF NEW BIOPREPARATIONS BASED ON AVERKOM ON DEVELOPMENT AND PRODUCTIVITY OF THE PLANTS AND ON EXPRESSION OF GENES OF si/miRNA SYNTHESIS

Abstract

Aim. To strengthen the resistance of cucumbers and a potato plants to nematodes by means of new effective microbic preparations on the basis of avermectines and to determine the molecular-genetic mechanisms of their protective action. Methods. Methods of field and molecular-genetic experiments, such as: isolation of si/miRNA from plant cells, the Dotblot hybridization si/miRNA with populations of cytoplasmic mRNA, investigation of functional activity si/miRNA in cell free system of protein synthesis. Results. In the field and greenhouse experiments at cucumbers and potato plants treated by new microbic preparations Averkom (producer strain Streptomyces avermitilis UKM Ас-2179) and its modifications the reduction of affection by parasitic nematodes and increase in productivity of cultures are determined. By Dot-blot method the difference (6–23%) is found in the degree of homology si/miRNA and mRNA between the experimental and control plants. In the cell free systems of protein synthesis from wheat sprouts the high level (38–65%) antinematode silencing activity of small regulatory si/miRNA from cells of experimental plants is confirmed. Conclusions. Increase of resistance to parasitic nematodes of the infected cucumbers and potato plants at the treatment by preparations on the basis of Averkom and the established differences in homology percent betweenpopulations of small regulatory si/miRNA, isolated from these plants, evidence that immunomodulating action of these preparations is occurred in the direction of induction in plant cells of antinematode si/miRNA synthesis, as a result their affection by parasitic nematodes decreases and productivity raises.