Abstract
Surfaces decorated with high aspect ratio nanostructures are a promising tool to study cellular processes and design novel devices to control cellular behavior. However, little is known about the dynamics of cellular phenomenon such as adhesion, spreading, and migration on such surfaces. In particular, how these are influenced by the surface properties. In this work, fibroblast behavior is investigated on regular arrays of 1 µm high polymer nanopillars with varying pillar to pillar distance. Embryonic mouse fibroblasts (NIH-3T3) spread on all arrays, and on contact with the substrate engulf nanopillars independently of the array pitch. As the cells start to spread, different behavior is observed. On dense arrays which have a pitch equal or below 1 µm, cells are suspended on top of the nanopillars, making only sporadic contact with the glass support. Cells stay attached to the glass support and fully engulf nanopillars during spreading and migration on the sparse arrays which have a pitch of 2 µm and above. These alternate states have a profound effect on cell migration rates. Dynamic F-actin puncta colocalize with nanopillars during cell spreading and migration. Strong membrane association with engulfed nanopillars might explain the reduced migration rates on sparse arrays.
Highlights
Surfaces decorated with high aspect ratio nanostructures are a promising of cell mechanotransduction machinery[23] or controlling the geometry of in vitro tool to study cellular processes and design novel devices to control cellular neuronal networks.[24]
To better understand the cell responses to HARNs and how they might be manipulated, we investigate the dynamics of embryonic mouse fibroblast (NIH-3T3) adhesion, spreading and migration on arrays of high aspect ratio polymer nanopillars as a function of array pitch
Nanopillar tip diameter is in the range of 90 nm, and the diameter at the base is 130 nm for the 1 μm high nanopillars used in this study
Summary
Surfaces decorated with high aspect ratio nanostructures are a promising of cell mechanotransduction machinery[23] or controlling the geometry of in vitro tool to study cellular processes and design novel devices to control cellular neuronal networks.[24]. Cells stay attached to the glass support and fully engulf was used as a nanomechanical biosensor to probe cell-induced forces by living cells with a resolution of 50 piconewton.[26] An attempt to control the geometry of in vitro cultivated neuronal cells revealed nanopillars during spreading and migration on the sparse arrays which have a that the cytoskeleton dynamics at the axon pitch of 2 μm and above. These alternate states have a profound effect on cell migration rates. An example of in vivo application is provided by Tang
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