Abstract
FeSO4 . 7 H2O, PbSO4, CuSO4 . 5 H2O, MnSO4 . 4 H2O, ZnSO4 . 7 H2O, CoCl2 . 6 H2O, and (NH4)6Mo7O24 . 4 H2O were added to the fermentation medium to investigate their potentialities on citric acid production by Candida lipolytica (Y 1095). FeSO4 . 7 H2O and MnSO4 . 4 H2O were more suitable than the other micronutrients for citric acid production. Addition of certain amino acids to the fermentation medium completely inhibited biosynthesis of citric acid by Candida lipolytica (Y 1095). When the fermentation medium was supplied with certain vitamins, yield of citric acid was increased in the presence of thiamine, nicotinic acid, and nicotinamide. The optimum concentration of thiamine which favoured yield of citric acid was 6 mg/l.
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