Abstract
Different strains of yeast were grown on different fermentation media for citric acid production. Candida lipolytica (Y-1095) produced a higher yield of citric acid than the other experimental yeasts. The best incubation period for the production of citric acid was found to be 144 h. Glycerol and glucose were the best carbon sources for citric acid production by Candida lipolytica (Y-1095). The optimum concentrations of glycerol and glucose were 80 and 120 g litre −1, respectively. The best organic and inorganic nitrogen sources were Hochest dry corn-steep, yeast extract, NH 4Cl and (NH 4) 2SO 4. The concentration of yeast extract and corn-steep powder (Hochest) which supported the greatest production of citric acid was 1·0 g litre −1, while the best concentrations of (NH 4) 2SO 4 and NH 4C were 2·0 g litre −1 and 1·5 g litre −1 respectively. KH 2PO 4 was the most suitable phosphorus source for citrici acid formation at a concentration of 1·5 g litre −1 and in the presence of MgSO 4.7H 2O at 1·2 g litre −1.
Published Version
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