Influence of methods of treatment of the periimplantit on chemiluminescent activity of neutrophil granulocytes in vitro.

Abstract

Abstract AIMS: Evaluation of the biocompatibility of implants removed from the site of inflammation and treated with air-flow and laser. MATERIALS AND METHODS: Three types of implant were investigated: anodized titanium dioxide surface (TiO2), coarse sandblasting and acid etching (Sand-blasted, Large grit, Acid-etched - SLA), RBM (Resorbable Blast Media). Implants were removed from the jaw of patients diagnosed with periimplantitis and the implant surface was treated with Air-flow air-abrasive mixture and chlorhexidine (first experimental group) and YSGG 2780 nm laser (second experimental group). New, out-of-package implants were used as controls. Biocompatibility was assessed by the level of synthesis of primary and secondary reactive oxygen species (ROS) by neutrophils, the intensity and kinetics of synthesis of which were determined by chemiluminescence analysis. RESULTS: Lucigenin- and luminol-dependent chemiluminescence of neutrophils was studied in vitro after incubation with SLA, RMB and TiO2 implants removed from the jaws of patients diagnosed with periimplantitis and treated with air-flow and chlorhexidine-air-abrasive mixture. It was found that, regardless of the type of implant studied, the time to reach maximum was reduced and the values of maximum intensity and area under the curve of spontaneous and zymosan-induced chemiluminescence of neutrophils were increased. The change in the values of zymosan-induced chemiluminescence activity indices of neutrophils during incubation with implants is much higher than the values of spontaneous chemiluminescence, leading to an increase in the values of the activation index. Incubation of neutrophils in vitro with laser-treated SLA, RMB and TiO2 implants does not cause significant changes in the values of neutrophil chemiluminescence indices. CONCLUSIONS: SLA, RMB and TiO2 implants removed from the jaws of patients with periimplantitis and treated with air-flow and chlorhexidine have a low biocompatibility. However, implants with the RBM surface after air-flow treatment have relatively higher biocompatibility in comparison with ..... продложение не влезло, находится в файле.