Abstract
Reproducible sporulation using solid-plate cultures ofStreptomyces coelicolor A3(2) was obtained on a defined medium with glucose as the primary source of carbon and sodium nitrate as the sole source of nitrogen. The type of agar used as a solidifying agent, and the inclusion of trace salts to the medium, significantly affected sporulation. An interrelationship between the medium carbon to nitrogen ratio and sporulation was observed, with clear promotion of spore formation under nitrogen-limited conditions, whilst carbon-limited conditions suppressed sporulation. The influence of medium phosphate upon sporulation is also reported, with high concentrations exerting an inhibitory effect.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
