Abstract

The California Mastitis Test was used as an indicator of mastitis in this study. Five cows were chosen for each of the five test scores (from 0=healthy to 4=severe mastitis). Milk samples were analyzed for free AA and free D-AA. The contents of free AA, free D-AA, and the ratio of free D-AA to free AA increased significantly as the California Mastitis Test score increased. The free D-AA content of foremilk (first milk jets) from healthy cows (test score=0) was approximately five times that in samples drawn later from the same udders. Contents of free AA and free D-AA were highly associated with test score and udder inflammation. Very low concentrations of free D-AA are normal for raw milk. Higher concentrations of free D-AA could be attributed to inclusion of foremilk and milk from cows having subclinical mastitis in the bulk tank milk.

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