Abstract
A low power laser has been used extensively in medical applications whereas many studies indicated important positive effects of laser therapy on a biological system. This research is designed to investigate in vitro the exposure of low-power diode pumping solid state (DPSS) laser influence on some rheological parameters, viscosity, and erythrocytes deformability on human red blood cells. Blood samples were obtained by venipuncture in tube-containing EDTA as an anticoagulant. Blood sample was separated into four equivalent aliquots. One aliquot served as control, and the remaining three aliquots were exposed to low power DPSS laser with wavelength 589 nm in continuous wave. The application of radiation has been used for (20, 30, and 45 min respectively) giving a power density of 15, 22.5, and 33.5 J/cm2 respectively. For non-radiated sample and for radiated samples after each given time, the viscosity of RBC suspension relative to phosphate buffered saline solution viscosity and erythrocytes deformability index (EDI) were measured. The comparison results showed upon exposure of RBC suspension to irradiation dose of 15, 22.5, 33.5 J/cm2 is associated with a significant reduction in viscosity of the suspension of the RBCs P < 0.0008, p < 0.0004, p < 0.0072 respectively. While the results of erythrocyte deformability showed significant differences on p < 0.02, p < 0.01, P < 0.03 respectively. It’s clear to notice that the optimum dose is 22. J/cm2 to induce maximum effect. It can be inferred that the viscosity of the RBC suspension was improved by laser irradiation, the deformability of blood samples increased as exposure time increased.
Published Version
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