Influence of Local Burning on Difference Reflectance Indices Based on 400-700 nm Wavelengths in Leaves of Pea Seedlings.

Ekaterina Sukhova,Anastasiia Ryabkova,Lyubov Yudina,Ekaterina Gromova,Vladimir Vodeneev,Vladimir Sukhov

doi:10.3390/plants10050878

Ekaterina Sukhova, Anastasiia Ryabkova + Show 4 more

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https://doi.org/10.3390/plants10050878

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Abstract

Local damage (e.g., burning) induces a variation potential (VP), which is an important electrical signal in higher plants. A VP propagates into undamaged parts of the plant and influences numerous physiological processes, including photosynthesis. Rapidly increasing plant tolerance to stressors is likely to be a result of the physiological changes. Thus, developing methods of revealing VP-induced physiological changes can be used for the remote sensing of plant systemic responses to local damage. Previously, we showed that burning-induced VP influenced a photochemical reflectance index in pea leaves, but the influence of the electrical signals on other reflectance indices was not investigated. In this study, we performed a complex analysis of the influence of VP induction by local burning on difference reflectance indices based on 400–700 nm wavelengths in leaves of pea seedlings. Heat maps of the significance of local burning-induced changes in the reflectance indices and their correlations with photosynthetic parameters were constructed. Large spectral regions with significant changes in these indices after VP induction were revealed. Most changes were strongly correlated to photosynthetic parameters. Some indices, which can be potentially effective for revealing local burning-induced photosynthetic changes, are separately shown. Our results show that difference reflectance indices based on 400–700 nm wavelengths can potentially be used for the remote sensing of plant systemic responses induced by local damages and subsequent propagation of VPs.

Highlights

Local actions of stressors on plants require systemic adaptive responses based on the generation and propagation of long-distance stress signals
It is shown that differences were absent in the reflectance indices (RIs) before variation potential (VP) induction
Photosynthetic processes are an important target of VP [2,3,5,7,8,27,28,30,31,37,38,39,40,41,42,43,44,45,48]

Summary

Introduction

Local actions of stressors on plants require systemic adaptive responses based on the generation and propagation of long-distance stress signals. It is known that an action potential is a self-propagating depolarization electrical signal [1,2,3,6,10] induced by non-damaging stimuli and is caused by both transient activation of Ca2+ , K+ , and anion channels, and inactivation of H+ -ATPase in the plasma membrane. The system potential is a weakly investigated hyperpolarization signal [8,11,12] caused by transient activation of H+ -ATPase and, possibly, changes in activity K+ channels. A VP is a long-distance signal in higher plants induced by local damage [2,4,6,13], which is formed by long-term depolarization and short-term “AP-like” spikes. The generation of a VP is mainly based on transient inactivation of H+ -ATPase, induced by Ca2+

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