Abstract
Water hyssop (Bacopa monnieri (L.) Pennell) is a medicinal plants. Its upper and lower halves of leaf explants were incubated in Murashige and Skoog (MS) medium supplemented with 0.25, 0.50 and 1.0 mg/L benzylaminopurine (BA) for 8 weeks; the explants were exposed to white (W) and red and blue (R and B, respectively) light-emitting diodes (LEDs), at 4:1, 3:1, 2:1 and 1:1 R and B light ratios, respectively. Shoot regeneration (100%) was achieved from all explants at all applied concentrations of BA and LED types. All explants showed different BA concentration requirements for regeneration of the maximum number of shoots. Longer shoots were obtained on medium with 0.25 mg/L BA. The W LED lighting system was found to be more effective for regenerating the maximum number of shoots (26.11) per explant (on the upper half of the leaf). Conversely, longer and shorter shoots were generated under 1:1 R:B and W LEDs, respectively. The number of shoots per explant ranged from 9.67-24.0 (full leaf), 6.33-25.92 (lower half of the leaf) and 7.33-27.33 (upper half of the leaf), respectively, in response to BA and LED light. Shoot length ranged from 0.94-1.90 cm (full lamina), 0.70-2.11 cm (lower half of the leaf) and 0.93-1.83 cm (upper half of the lamina) in response to BA and LED lifght. Regenerated shoots were successfully rooted using indole-3-butyric acid (IBA) and acclimatized in the aquarium provided with tap water.
Highlights
Water hyssop (Bacopa monnieri (L.) Pennell) or Brahmi is a well-known Indian plant grown in damp and marshy areas [1,2]
The aim of the present study was to show the potential of different combinations of red (R) and blue (B) Light-emitting diodes (LEDs), as compared to white (W) LEDs, and their effect on shoot regeneration obtained from different leaf explants of water hyssop
Direct shoot induction started within 8-10 days from the margins of leaf explants (Fig. 2a and b) followed by shoot bud induction and shoot regeneration within three weeks (Fig. 2 c)
Summary
Water hyssop (Bacopa monnieri (L.) Pennell) or Brahmi is a well-known Indian plant grown in damp and marshy areas [1,2]. Because of overexploitation the Water hyssop is a threatened and endangered species [1,11] High demand for this plant is a challenge for researchers to develop new regeneration protocols in order to conserve plants and offer an alternative routes of production, e.g. by in vitro propagation[3,12,13,14,15,16].
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