Influence of Jagged1 on apoptosis-related gene expression: a microarray database analysis

Abstract

Notch signaling regulates various cell behaviors including cell death and survival. The present study aimed to investigate the influence of Notch ligand, Jagged1, on the expression of apoptosis related genes using the microarray database analysis. In this regard, microarray dataset from Gene Expression Omnibus (GEO) repository was searched with keywords. The included GEO series were downloaded and analyzed using the Connection Up- and Down-Regulation Expression Analysis of Microarrays Extension program. The predicted protein–protein interaction was determined using GenesFANs program. The results demonstrated that Jagged1 enhanced pro-apoptosis gene expression in human lung and breast cancer cell line while promoted the expression of anti-apoptosis gene in human lymphatic epithelial cells. In human umbilical endothelial vein cells, Jagged1 promoted both pro- and anti-apoptosis gene expressions. No significant change in apoptotic related gene was noted in Jagged1 overexpressing human endometrial stromal cells. However, Jagged1 down-regulated both pro- and anti-apoptosis gene in human lung and breast cancer cell line as well as human umbilical endothelial vein cells. In conclusion, Jagged1 regulated both pro- and anti-apoptosis gene expressions, depending on cell types. However, the influence of Jagged1 on cell apoptosis or survival is needed further confirmation experiments.