Abstract
Summary The effect of assay medium pH on in vivo nitrate reductase (NR) activity of cashew ( Anacardium occidentale L.) leaves was studied. The enzyme activity continued to increase beyond pH 7.5 up to pH 10 until the leaf discs showed symptoms of damage. Entry of nitrate into the leaf tissue also increased up to pH 9. Higher activities of NR at alkaline pH were also observed in cacao, country bean ( Dolichos lablab ) and sugarcane leaves. This is perhaps the first time that such a high range is reported as optimal pH for NR in higher plants.
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