Abstract
Incubation of bovine spermatozoa at 25° C for up to 8 hours in seminal plasma had little influence on oxygen uptake as compared to preincubation values but increased both the number of dead spermatozoa and proportion of spermatozoa with detached acrosomes. Washing spermatozoa and resuspension in either saline or seminal plasma followed by incubation for 4 or 8 hours decreased oxygen uptake and at 8 hours decreased the proportion of cells with deteched acrosomes as compared to 8 hour control values. When spermatozoa were not washed but were extended in egg yolk citrate extender or seminal plasma, oxygen uptake was greater for cells extended with seminal plasma. Storage of spermatozoa for 14 days at −196° C following incubation in seminal plasma showed little influence of incubation time in seminal plasma on postfreeze metabolic or morphological characteristics. These experiments indicate that incubation of spermatozoa in seminal plasma prior to storage has little beneficial effect and, on the contrary, may cause an increase in acrosomal loss.
Published Version
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