Abstract

AbstractWe demonstrated theoretically and experimentally the influence of polarization of an input light beam on the intensity and polarization distributions of a tight focusing spot, obtained by a high numerical aperture (NA) objective lens (OL). The numerical calculations, based on vectorial Debye theory, show that, under tight focusing condition (NA = 1.4), the shape of the focusing spot varies as a function of the input beam polarization, resulting in either asymmetric or symmetric spots. A home made confocal microscope constituted by a high NA OL is employed to scan the fluorescence image of single gold nanoparticles showing the actual polarization dependence of focusing spot. The input linear polarization allows generating a small but asymmetric focusing spot, while the circular polarization produces a larger but symmetric one.

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