Abstract
The influence of an insufficient supply of H 2 on microbial gas consumption was investigated in autotrophic culture of Alcaligenes eutrophus ATCC 17697 T. Experiments were conducted in a recycled-gas, closed-circuit system consisting of a 200-ml vessel containing 100-ml culture of 8.0 g· l −1 (on a dry matter basis) viable cells. A mixed gas of CO 2, H 2 and O 2 was used, in which the partial pressures of CO 2 and O 2 were kept at 10.1 and 20.3 kPa respectively, but that of H 2 was varied from 70.9 to nearly zero kPa by mixing with N 2. The total pressure was maintained at 101.3 kPa. The gas mixture was fed into the vessel at a flow rate of 100 ml·min −1 and the gas consumption rates of CO 2, H 2 and O 2 as well as the partial pressures of dissolved H 2 and O 2 ( p LH and p LO) were determined. The dissolved H 2 concentration ( C H) was calculated from the p LH, and its relationship with each gas consumption rate was examined. These values were plotted on a hyperbola. Lineweaver-Burk plots of all the gas consumption rates against C H gave straight lines. Gas consumption rates during the growth of this microorganism could be changed by varying the p LH under H 2-limited conditions, and could be expressed in the same form as Monod's equation with C H as the sole variable.
Published Version
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