Influence of Human Jaw Periosteal Cells Seeded β-Tricalcium Phosphate Scaffolds on Blood Coagulation.

Marbod Weber,Hans-Peter Wendel,Siegmar Reinert,Lin Tzu Yu,Felix Umrath,Heidrun Steinle,Dorothea Alexander,Meltem Avci-Adali,Lukas-Frank Schmitt,Christian Schlensak

doi:10.3390/ijms22189942

Marbod Weber, Hans-Peter Wendel + Show 8 more

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https://doi.org/10.3390/ijms22189942

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Abstract

Tissue engineering offers auspicious opportunities in oral and maxillofacial surgery to heal bone defects. For this purpose, the combination of cells with stability-providing scaffolds is required. Jaw periosteal cells (JPCs) are well suited for regenerative therapies, as they are easily accessible and show strong osteogenic potential. In this study, we analyzed the influence of uncoated and polylactic-co-glycolic acid (PLGA)-coated β-tricalcium phosphate (β-TCP) scaffolds on JPC colonization and subsequent osteogenic differentiation. Furthermore, interaction with the human blood was investigated. This study demonstrated that PLGA-coated and uncoated β-TCP scaffolds can be colonized with JPCs and further differentiated into osteogenic cells. On day 15, after cell seeding, JPCs with and without osteogenic differentiation were incubated with fresh human whole blood under dynamic conditions. The activation of coagulation, complement system, inflammation, and blood cells were analyzed using ELISA and scanning electron microscopy (SEM). JPC-seeded scaffolds showed a dense cell layer and osteogenic differentiation capacity on both PLGA-coated and uncoated β-TCP scaffolds. SEM analyses showed no relevant blood cell attachment and ELISA results revealed no significant increase in most of the analyzed cell activation markers (β-thromboglobulin, Sc5B-9, polymorphonuclear (PMN)-elastase). However, a notable increase in thrombin-antithrombin III (TAT) complex levels, as well as fibrin fiber accumulation on JPC-seeded β-TCP scaffolds, was detected compared to the scaffolds without JPCs. Thus, this study demonstrated that besides the scaffold material the cells colonizing the scaffolds can also influence hemostasis, which can influence the regeneration of bone tissue.

Highlights

IntroductionThe repair of small and large bone defects is of key interest in oral and maxillofacial surgery
Jaw periosteal cells (JPCs) cultivated on polylactic-co-glycolic acid (PLGA)-coated β-tricalcium phosphate (β-TCP) scaffolds without osteogenic induction at days 6 and 13 showed a slightly lower cell viability compared to the JPCs on uncoated scaffolds (Figure 1C)
We demonstrated that uncoated and PLGA-coated β-TCP scaffolds are highly suitable for the colonization with JPCs and their further osteogenic differentiation

Summary

Introduction

The repair of small and large bone defects is of key interest in oral and maxillofacial surgery. Molecular, as well as cell-based therapies are applied for the engineering of new bone tissue. Mesenchymal stromal cells (MSCs) derived from bone marrow have been used for regenerative bone formation [1,2]. These cells have a multipotential differentiation capacity into osteoblasts, adipocytes, and chondrocytes [3,4]

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