Abstract
Summary Fourteen ejaculates, two from each of seven bulls, were extended in heated, whole homogenized milk (milk) and egg yolk-citrate (EYC), frozen with either dry ice-alcohol (DI) or liquid nitrogen (LN), then divided for storage at −79C (DI), −92C (Mechanical), and −196C (LN). Samples from each ejaculate and each treatment were evaluated for pH and per cent progressive motility at prefreeze, post-freeze, one day, ten days, one, three, six, and nine months. EYC extender maintained significantly higher (P There was little variation in mean pH values within extenders due to freezing methods or storage temperatures. The correlation coefficients between pH and motility and between change in pH and change in motility were low and in most instances nonsignificant.
Published Version
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