Abstract
Garlic (Allium sativum L) is one of genus Allium sp which propagated vegetatively. In vegetative propagation, virus is one of the major diseases. However, it has been approved that OYDV (Onion Yellow Dwarf Virus) can be eliminated through unconventional propagation techniques. The aim of this research was to evaluate the effect of explant, variety and heat treatment on the growth and development of explants dealing with virus elimination. The research was conducted on the IVEGRI tissue culture laboratory from May to September 2018. The research was designed using combination between explant types e.g. meristem (E1) and shoot tip (E2), heat treatment (370C) for 1 month, and varieties e.g. Lumbu Hijau (V1); Tawang Mangu (V2); Lumbu Putih (V3). The composition of media consisted of MS + MS vitamine + Sucrose 30 g L-1+ IAA 2 mg L-1 + Kinetin 2 mgL-1 + GA30.01 mgL-1 + Gel gro 2 gL-1, pH 5.7 were used as media growth. The results concluded that explant size, heat treatment and varieties affected percentage of proliferation. The bigger the explant size used, the higher growth percentage resulted. From the average number of shoots, leaves and roots, it also suggested that explant type and varieties affected the explant growth and development. Moreover, it was shown that the culture contamination on 10 WAP ranged from 20% to 55% which generally caused by bacteria. The results of virus testing OYDV on plantlets using DAS ELISA Serology technique showed that the infected cultures ranged from 22.22% to 60 %.
Highlights
The Garlic (Allium sativum L) belongs to genus Allium which is propagated vegetatively
An alternative way to remove/eliminate viruses can be performed by heat treatment from a donor explant or planting different type of explant such as meristematic tissue
The aim of this research was to evaluate the effect of explant type, variety and heat treatment on the growth and development of explants to produce disease-free plants
Summary
The Garlic (Allium sativum L) belongs to genus Allium which is propagated vegetatively. Garlic propagation can be carried out through unconventional or micropropagation method by tissue culture techniques. Goal of unconventional propagation is to improve quality or propagate plant organ [1,2,3]. Viral systemic disease is one of the important diseases that must be eliminated from the plant that propagated vegetatively. Research of Walkey et al [4] and Fayek et al [5], indicated that this systemic disease infection can reduce production between 2 - 50%. An alternative way to remove/eliminate viruses can be performed by heat treatment from a donor explant or planting different type of explant such as meristematic tissue
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call