Abstract
Due to the high prevalence of death on the base of alcoholic intoxication of varying degrees, it is expedient to analyze the possibility of the influence of alcohol on the change in the structure of the polycrystalline films of the cerebrospinal fluid, and accordingly on the accuracy of the diagnosis of the time since death by the method of autofluorescent laser polarimetry.The purpose of the work. To study the possible influence of alcohol and to develop objective forensic criteria for diagnosing its effects on the basis of analysis of changes in the polycrystalline structure of cerebrospinal fluid (CSF) films. Materials and methods. Object of research: native films of CSF, selected in 80 corpses, in the quantity of 155 samples - 1 group; CSF from 30 healthy volunteers (group 0) - 30 samples; CSF films obtained in the deceased with the presence of ethyl alcohol in the blood, 30 samples - group 2. The methods of gas-liquid chromatography, laser-induced fluorescence and statistical processing of the results were used. Results. The efficiency of using the methods of laser autofluorescence of polycrystalline films of the cerebrospinal fluid for the diagnosis of the time since death was proved. A statistically significant difference between cases of death from cardiovascular pathology with the presence and absence of ethyl alcohol in the organism was established by determining the magnitude of the statistical moment of the 4th order, which characterizes distribution of azimuth polarization values of microscopic images of laser-induced fluorescence. On this basis, an excellent balanced accuracy of the differentiation of the presence of ethyl alcohol in the human body (Ac (M4) = 94%) was achieved. Conclusion. Ethyl alcohol affects the structure of the polycrystalline films of the cerebrospinal fluid, which must be taken into account when estimating the time since death by autofluorescence laser polarimetric methods.
Highlights
New information on the morphological and optic-anisotropic structure of biological objects, which makes it possible to establish a connection between their physiological state and polarization -phase parameters of the images of their architectonics [6], that is why it is advisable to assume their sensitivity and to detect alcohol in the cerebrospinal fluid (CSF) of deceased persons
As research objects in work were used 3 groups: group 1 - native preparations of CSF films, selected from 80 corpses of both sexes, who died of pathology of the circulatory system in the age of 33 to 89 years, with a previosly known time of death, ranging from 1 to 26 hours - 155 specimens; group 2 - posthumous polycrystalline planar films of CSF, obtained from persons that have died from the pathol- ogy of blood circulatory system with the presence of ethanol in the blood - 30 samples; group 0 - 30 healthy volunteers - 30 samples
Concentration of ethyl alcohol in blood was determined during the forensic medical examination of corpses in the department of forensic medical toxicology of the CMI “Regional Bureau of Forensic Medical Examination” of the Department of Healthcare of Chernivtsi Regional State Administration through gas-liquid chromatography on the device “Crystal-2000M”
Summary
1. The possibilities of forensic medical objective differentiation of the estimation of the influence of ethyl alcohol on the course of post-mortem processes in case of cardiovascular pathology by the method of autofluorescence microscopy of polycrystalline films of the cerebrospinal fluid were analyzed. Stable laser polarimetry of polycrystalline films of human biological fluids. Сучасний алгоритм судово-медичної діагностики давності настання смерті у ранній постмортальний період. Вплив алкоголю на динаміку імунних показників крові померлих від ішемічної хвороби серця (ІХС) при встановленні давності настання смерті. Судово-медична діагностика давності настання смерті за суправітальною реакцією зіниць [автореферат]. Post mortem interval estimation: features of cerebrospinal fluid films autofluorescent laser polarimetry.
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